Center for Air and Aquatic Resources Engineering and Sciences (CAARES)
About CAARES
Learn more about our research center's efforts and collaborations:
DoD-ELAP Accreditation
CAARES currently holds an ISO/IEC 17025:2017 and US Department of Defense Environmental Laboratory Accreditation Program certification for testing of PFAS using EPA Method 1633. CAARES routinely analyzes samples under the QA/QC requirements of this standard to produce high-quality data to support in-house research and external clients.
CAARES Equipment and Facilities
CAARES has analytical facilities that house a number of major instruments, as well as laboratory space specifically designed to support research. The analytical
instrumentation is capable of analyzing for polar and non-polar organics along with
metals and elements. The major types of instruments include liquid chromatography-
mass spectrometry (LC-MS), Gas Chromatography-Mass Spectrometry (GC-MS,
inductively coupled plasma-mass spectrometry (ICP-MS) and Ion Chromatography
(IC)/Combustible Ion Chromatography (CIC) systems.
Capabilities
- A Thermo Vanquish UPLC coupled to an Altis Triple Quadrupole Mass Spectrometer is available for targeted analysis of polar organic compounds in ESI or APCI modes. Instrument is typically used in Multiple Reaction Monitoring (MRM) mode for targeted analysis of polar organic contaminants including PFAS.
- A Waters Xevo Absolute Triple Quadrupole LC-MS system is available for the targeted analysis of polar organic contaminants including PFAS.
- A Sciex X500B Quadrupole Time-of-Flight (QToF) High Resolution Mass Spectrometry is available for accurate mass measurement (>20,000 res). The instrument can be used for both small (contaminants, metabolites) and large (polymers, proteins) molecule analysis.
Single and Tandem Mass Spectrometry
An Agilent 7890GC coupled to a 5977A MSD (single quadruple) is available for targeted analysis of volatile compounds. This instrument can be used coupled to a liquid injector or to a headspace sampler. A Waters Xevo TQ-XS triple quadrupole system is available for targeted analysis using MRM mode. The instrument is coupled to a GC using an atmospheric pressure chemical ionization source which is a soft ionization technique that creates stable molecular ions compared to traditional electron ionization. Legacy contaminants such as Dioxins/Furans, PCBs, PCNs and OCPs are routinely analyzed using this system.
Two-Dimensional Gas Chromatography-High Resolution Mass Spectrometry (GCxGC-HRMS)
A GCxGC-HRMS system (LECO Pegasus GC-HRT + 4D system) is utilized for the non- targeted analysis of volatile contaminants in complex environmental media. This state- of-the-art system combines enhanced chromatographic separation with accurate mass measurement to help identify novel contaminants. The instrument can be operated in both electron ionization and chemical (positive/negative) ionization modes.
A Thermo iCAP-RQ ICP-MS system is available for elemental/metals analysis. The
instrument can be operated in kinetic energy discrimination (KED) mode to remove
interfering polyatomic species. The instrument is used for both environmental and
biological sample analysis.
A Thermo EXTREVA accelerated solvent extraction (ASE) system is available for the extraction of a wide range of samples. This fully automated system can be used for in-cell cleanup and evaporation of extracts.
Automated SPE systems are available for cleanup of legacy contaminants including PCBs, Pesticides and Dioxins as well as emerging contaminants such as PFAS.
A Thermo Integrion IC is available for the analysis of cations and anions. A CIC from
Metrohm is available for Fluorine measurement including extractable organic fluorine
(EOF).
A Milestone DMA-80 is available for mercury analysis. Biological samples such as fish tissue and hair are routinely analyzed.
Our DIONEX ASE300 Accelerated Solvent Extraction system prepares organic samples for processing. This unit uses elevated temperature and pressure, permitting rapid extraction of the samples with a much smaller volume of solvent than a conventional Soxhlet extraction system.
The lab also has a Fluid Management Systems (FMS) PowerPrep SPE Automated One-Step Extraction and Concentration System and an automated gel permeation chromatography (GPC) system for handling multiple samples simultaneously.
A Shimadzu LC-2040C HPLC is available with a photodiode array (PDA) detector. The instrument is used for analysis of polar organic compounds.
Faculty and Staff
Many faculty from different departments collaborate with CAARES. In addition, we work closely with the Institute for a Sustainable Environment, where we also have faculty appointed.
